• Chemistry and Materials

Miniaturisation for Synthesis, Analysis and Proteomics (MSAP)

Research unit - USR 3290

Description

MSAP is a service and research unit that is at the interface of chemistry and biology. We are well recognized internationally for our instrumental and analytical developments related to mass spectrometry and we have built a solid reputation in the field of art analysis or identification of biological macromolecules. In chemistry, the originality of our unit is based on flow synthesis (from milli- to nano-fluidic) with developments in photochemistry or nanoparticle synthesis. MSAP also includes a mass spectrometry platform as well as a proteomics platform, and benefits from access to state-of-the-art equipment. We are open to all academic and industrial collaborations and propose services in line with our expertise.

Contacts

  • Stéphanie Flament
  • Ahmed Mazzah
    Director

Informations

Bâtiment C4, Avenue Paul Langevin
Campus Cité Scientifique, Université de Lille
59655 VILLENEUVE D’ASCQ

http://msap-lab.fr

Miniaturisation for Synthesis, Analysis and Proteomics

Effectif

Effectif total : 16

Personnel d'appui à la recherche : 4

Expertises

Skills

• Mass spectrometry
• Proteomics
• Post-translational modifications (phosphorylation, glycosylation ...)
• Instrumental development
• Analytical developments: biomolecules, biobased
• Development of microreactors in organic synthesis
• Flow chemistry
• Chromatography
• Separation techniques
• Fine chemistry in multiscale microfluidic device
• Synthesis of nanoparticles
• Organic physico-chemistry
• Photochemistry

Example(s) of projects

• Actinometric analysis of compounds
• Multi-scale analysis of yeasts and their hydrolysates, from peptides to proteins
• Study of the works of Amadeo MODIGLIANI (LaM Lille Métropole Musée d'Art Moderne) 🡭

Example(s) of publications

• Colpaert, M., Kadouche, D., Ducatez, M., Pillonel, T., Kebbi-Beghdadi, C., Cenci, U., … & Colleoni, C. (2021). Conservation of the glycogen metabolism pathway underlines a pivotal function of storage polysaccharides in Chlamydiae. Communications biology, 4(1), 1-16. 🡭
• Venzac, B., Deng, S., Mahmoud, Z., Lenferink, A., Costa, A., Bray, F., … & Le Gac, S. (2021). PDMS Curing Inhibition on 3D-Printed Molds: Why? Also, How to Avoid It?. Analytical Chemistry. 🡭
• Le Guen, C., Mazzah, A., Penhoat, M., Melnyk, P., Rolando, C., & Chausset-Boissarie, L. (2021). Direct and Regioselective C (sp3)–H Bond Fluorination of 2-Alkylazaarenes with Selectfluor. Synthesis, 53(06), 1157-1162. 🡭
• Jóźwiak, P.; Ciesielski, P.; Zakrzewski, P.K.; Kozal, K.; Oracz, J.; Budryn, G.; Żyżelewicz, D.; Flament, S.; Vercoutter-Edouart, A.-S.; Bray, F.; Lefebvre, T.; Krześlak, A. Mitochondrial O-GlcNAc Transferase Interacts with and Modifies Many Proteins and Its Up-Regulation Affects Mitochondrial Function and Cellular Energy Homeostasis. Cancers 2021, 13, 2956. 🡭
• Mustapha Najib, Fabrice Bray, Simon Khelissa, Stephanie Flament, Elodie Richard, Khaled El Omari, Christian Rolando, Guillaume Delaplace, Monzer Hamze, Nour-Eddine Chihib. Effect of milk heat treatment on the molecular interactions during the process of Qishta-a Lebanese dairy product. International Dairy Journal 2021. 🡭

Discover the full list of publications here.

Collaborations/Partners/Scientific clients

University of Louvain (Belgium), University of Quebec (Canada), University of Gent (Belgium), University of Hong Kong (Hong Kong)

Collaborations/Partners/Private Clients

Roquette, Lesaffre, Mäder Group...

Services offers

Applications sectors

  • Health / wellness
  • Art / Culture
  • Education / Training
  • Science / Research

Services provided

• Identification of biological macromolecules
• Analysis of works of art
• Organic & bioorganic analysis of cultural heritage samples
• Proteomic, lipidomic, glycomic analysis
• Development of microfluidic devices
• Lipid analysis with identification of structures by fragmentation in CID or IRMPD
• Top-Down analyzes with nanochromatographic coupling
• Identification, quantification and analysis of proteins
• Analysis of post-translational modifications (phosphorylation, glycosylation ...)
• Enrichment in phospho or glycopeptides / proteins with the use of custom columns
• Analysis of lipids such as triacylglycerols or metabolomics

Consulting services

We provide consulting services related to all our areas of expertise.

Equipments

We possess a wide range of equipment regrouped within the internal and external technological platforms comprising equipment for sample preparation, for proteomic analysis and by mass spectrometry, for data analysis. We also have:
• Mass spectrometers
• Nano-HPLC

See the detailed list of equipment: https://msap-lab.fr/instruments/

Ecosystem

Affiliated institutions / organisations

University of Lille
CNRS

Groups/Networks/Federations

FR 3624 « Spectrométrie de masse FT-ICR à très haut champ »
GIS IBiSA
EU_FT-ICR_MS - European Network of Fourier-Transform Ion-Cyclotron-Resonance Mass Spectrometry Centers
IPERION-HS
GDR Synthèse en Flux

Doctoral schools

Competitive cluster

Regional strategic areas of activity

  • Chemistry and Materials

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Thematics

Current thematics

  • Chemistry and Materials
Title

Current content

Miniaturisation for Synthesis, Analysis and Proteomics
Type

Current content

Research unit
Description

Current content

MSAP is a service and research unit that is at the interface of chemistry and biology. We are well recognized internationally for our instrumental and analytical developments related to mass spectrometry and we have built a solid reputation in the field of art analysis or identification of biological macromolecules. In chemistry, the originality of our unit is based on flow synthesis (from milli- to nano-fluidic) with developments in photochemistry or nanoparticle synthesis. MSAP also includes a mass spectrometry platform as well as a proteomics platform, and benefits from access to state-of-the-art equipment. We are open to all academic and industrial collaborations and propose services in line with our expertise.
Contact informations
Contact

Current content

Contact name Email phone number
FLAMENT Stéphanie stephanie.flament@univ-lille.fr (+33) 3 20 33 71 12
MAZZAH Ahmed msap-plateformes@univ-lille.fr (+33) 3 20 33 64 29
URL

Current content

http://msap-lab.fr

Address

Current content

Bâtiment C4, Avenue Paul Langevin
Campus Cité Scientifique, Université de Lille
59655 VILLENEUVE D’ASCQ
Keywords

Current content

Flow chemistry, Mass spectrometry, Proteomics, Cultural heritage, Miniaturization, Chromatography, Separation techniques, Synthesis, Organic physico-chemistry
Our search domains
Skills

Current content

• Mass spectrometry • Proteomics • Post-translational modifications (phosphorylation, glycosylation ...) • Instrumental development • Analytical developments: biomolecules, biobased • Development of microreactors in organic synthesis • Flow chemistry • Chromatography • Separation techniques • Fine chemistry in multiscale microfluidic device • Synthesis of nanoparticles • Organic physico-chemistry • Photochemistry
Workforce

Current content

Nombre de chercheurs :
Nombre de doctorants :
Nombre de personnel d'appui à la recherche : 4
Effectif total : 16
Example(s) of projects

Current content

• Actinometric analysis of compounds • Multi-scale analysis of yeasts and their hydrolysates, from peptides to proteins • Study of the works of Amadeo MODIGLIANI (LaM Lille Métropole Musée d'Art Moderne) 🡭
Example(s) of publications

Current content

• Colpaert, M., Kadouche, D., Ducatez, M., Pillonel, T., Kebbi-Beghdadi, C., Cenci, U., … & Colleoni, C. (2021). Conservation of the glycogen metabolism pathway underlines a pivotal function of storage polysaccharides in Chlamydiae. Communications biology, 4(1), 1-16. 🡭 • Venzac, B., Deng, S., Mahmoud, Z., Lenferink, A., Costa, A., Bray, F., … & Le Gac, S. (2021). PDMS Curing Inhibition on 3D-Printed Molds: Why? Also, How to Avoid It?. Analytical Chemistry. 🡭 • Le Guen, C., Mazzah, A., Penhoat, M., Melnyk, P., Rolando, C., & Chausset-Boissarie, L. (2021). Direct and Regioselective C (sp3)–H Bond Fluorination of 2-Alkylazaarenes with Selectfluor. Synthesis, 53(06), 1157-1162. 🡭 • Jóźwiak, P.; Ciesielski, P.; Zakrzewski, P.K.; Kozal, K.; Oracz, J.; Budryn, G.; Żyżelewicz, D.; Flament, S.; Vercoutter-Edouart, A.-S.; Bray, F.; Lefebvre, T.; Krześlak, A. Mitochondrial O-GlcNAc Transferase Interacts with and Modifies Many Proteins and Its Up-Regulation Affects Mitochondrial Function and Cellular Energy Homeostasis. Cancers 2021, 13, 2956. 🡭 • Mustapha Najib, Fabrice Bray, Simon Khelissa, Stephanie Flament, Elodie Richard, Khaled El Omari, Christian Rolando, Guillaume Delaplace, Monzer Hamze, Nour-Eddine Chihib. Effect of milk heat treatment on the molecular interactions during the process of Qishta-a Lebanese dairy product. International Dairy Journal 2021. 🡭 Discover the full list of publications here.
Collaborations/Partners/Scientific clients

Current content

University of Louvain (Belgium), University of Quebec (Canada), University of Gent (Belgium), University of Hong Kong (Hong Kong)
Collaborations/Partners/Private Clients

Current content

Roquette, Lesaffre, Mäder Group...
Services offers
Training offers

Current content

Services offers

Current content

• Identification of biological macromolecules • Analysis of works of art • Organic & bioorganic analysis of cultural heritage samples • Proteomic, lipidomic, glycomic analysis • Development of microfluidic devices • Lipid analysis with identification of structures by fragmentation in CID or IRMPD • Top-Down analyzes with nanochromatographic coupling • Identification, quantification and analysis of proteins • Analysis of post-translational modifications (phosphorylation, glycosylation ...) • Enrichment in phospho or glycopeptides / proteins with the use of custom columns • Analysis of lipids such as triacylglycerols or metabolomics
Consulting services

Current content

We provide consulting services related to all our areas of expertise.
Equipments
Equipements spécifiques

Current content

  • Mass spectrometer FT-ICR
    SolariX XR 9.4 Tesla/ Bruker
  • NanoESI Q-Orbitrap mass spectrometer
    Q-Exactive plus/ Thermo Scientific
  • Mass spectrometer LTQ
    Orbitrap XL / Thermo Scientific
  • Nano-HPLC chromatography
    U3000 RSLC/ Thermo Scientific
  • Mass spectrometer MALDI TOF/TOF
    4800+/ Absciex
Ecosystem
Affiliated institutions / organisations

Current content

  • University of Lille
  • CNRS
Établissement(s) partenaire(s)

Current content

Unit(s) of attachment

Current content

Groups/Networks/Federations

Current content

FR 3624 « Spectrométrie de masse FT-ICR à très haut champ » GIS IBiSA EU_FT-ICR_MS - European Network of Fourier-Transform Ion-Cyclotron-Resonance Mass Spectrometry Centers IPERION-HS GDR Synthèse en Flux
Doctoral schools

Current content

Equipex/EquipEx+/ESR

Current content

Competitive cluster

Current content

Regional strategic areas of activity

Current content

  • Chemistry and Materials
Personal informations
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