• Health and Nutrition

Laboratory of Cell Physiology (PhyCell)

Research unit - U 1003

Description

The scientific project of our laboratory encompasses fundamental aspects of ion channels’ biology with a particular focus on the consequences of their dysregulation during the development of prostate, pancreatic and colon cancers. Our experimental approaches include electrophysiology, imaging, biochemistry, molecular biology and flow cytometry techniques, validated in novel in vivo models. Our understanding of ion channels and some associated inflammasomes in pathological conditions allows us to develop biomarkers and innovative therapeutic approaches for a better management of "oncochannelopathies". Given the molecular complexity, tumor heterogeneity as well as resistance to anti-cancer therapies, we propose a strategy focused on ion channels and associated inflammasomes. This strategy has three components: (1) Identify a molecular "signature" that can be used for the development of a diagnostic/prognostic test for efficacy/toxicity to locoregional therapies in close collaboration with clinicians. (2) Develop the visualization and measurement of dynamic alterations of channels and inflammasomes during carcinogenesis. (3) Improve the current personalized medicine strategy against prostate, pancreatic and colorectal cancers.

Contacts

  • Sandy Despeghel
  • Natacha Prevarskaya
    Director

Informations

Bâtiment SN3, Avenue Paul Langevin
Campus Cité Scientifique, Université de Lille
59655 VILLENEUVE D’ASCQ

https://phycell.univ-lille.fr/

Laboratory of Cell Physiology

Effectif

Effectif total : 47

Personnel de recherche : 22

Personnel d'appui à la recherche : 8

Expertises

Skills

• Ion channels
• Single channels
• Ion channel pharmacology
• Intracellular signaling
• Calcium signaling
• Prostate cancer
• Pancreatic cancer
• Resistance to chemotherapy

Example(s) of projects

• Role of ion channels in tumor initiation and progression.
• TRPV6 regulation and targeting in cancer.
• Calcium-permeable channels in cancer-related senescence.

Example(s) of publications

• TRPM8 inhibits endothelial cell migration via a non-channel function by trapping the small GTPase Rap1. Genova T, Grolez GP, Camillo C, Bernardini M, Bokhobza A, Richard E, Scianna M, Lemonnier L, Valdembri D, Munaron L, Philips MR, Mattot V, Serini G, Prevarskaya N, Gkika D, Pla AF. J Cell Biol. 2017 Jul 3;216(7):2107-2130. doi: 10.1083/jcb.201506024. 🡭
• The calcium-cancer signalling nexus. Monteith GR, Prevarskaya N, Roberts-Thomson SJ. Nat Rev Cancer. 2017 Jun;17(6):367-380. doi: 10.1038/nrc.2017.18. 🡭
• Epidermal TRPM8 channel isoform controls the balance between keratinocyte proliferation and differentiation in a cold-dependent manner. Bidaux G, Borowiec AS, Gordienko D, Beck B, Shapovalov GG, Lemonnier L, Flourakis M, Vandenberghe M, Slomianny C, Dewailly E, Delcourt P, Desruelles E, Ritaine A, Polakowska R, Lesage J, Chami M, Skryma R, Prevarskaya N. Proc Natl Acad Sci U S A. 2015 Jun 30;112(26):E3345-54. doi: 10.1073/pnas.1423357112. 🡭
• TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity. Gkika D, Lemonnier L, Shapovalov G, Gordienko D, Poux C, Bernardini M, Bokhobza A, Bidaux G, Degerny C, Verreman K, Guarmit B, Benahmed M, de Launoit Y, Bindels RJ, Fiorio Pla A, Prevarskaya N. J Cell Biol. 2015 Jan 5;208(1):89-107. doi: 10.1083/jcb.201402076. 🡭
• Remodeling of channel-forming ORAI proteins determines an oncogenic switch in prostate cancer. Dubois C, Vanden Abeele F, Lehen'kyi V, Gkika D, Guarmit B, Lepage G, Slomianny C, Borowiec AS, Bidaux G, Benahmed M, Shuba Y, Prevarskaya N. Cancer Cell. 2014 Jul 14;26(1):19-32. doi: 10.1016/j.ccr.2014.04.025. 🡭

Collaborations/Partners/Scientific clients

National:
University de Valenciennes et du Hainaut-Cambresis, Institut Pasteur Lille, Centrale Lille, INSA Lyon, University Claude Bernard Lyon, CHU Lille, University of Paris, University of Tours, University of Grenoble Alpes, University of Clermont Auvergne
International:
Institute of Physiology Bogomoletz (Ukraine), University of Turin (Italy), University of Münster (Germany)

Services offers

Applications sectors

  • Health / wellness
  • Science / Research

Services provided

• Electrophysiological study of ion channels (whole cell, single channels...)
• Screening of pharmacological molecules and antibodies modulating ion channels
• Study of signaling pathways
• Calcium imaging

Consulting services

We provide consulting services related to all our areas of expertise.

Equipments

Our laboratory is equipped with:
- 5 patch-clamp stations
- a "Port-A-Patch" system (Nanion)
- two calcium imaging stations
- 2 confocal microscopes
- as well as basic laboratory equipment (cell culture equipment, cell and molecular biology, etc.).

Ecosystem

Affiliated institutions / organisations

Inserm
University of Lille

Partner institution(s)

Université catholique de Lille

Groups/Networks/Federations

GIS ONCOLille
ITN pHioniC

Equipex/EquipEx+/ESR

Doctoral schools

Regional strategic areas of activity

  • Health and Nutrition
    • Food ingredients
    • New therapeutic approaches

Request for modification

You enter the « edit request » mode. After sending your modifications, they will first be validated by the Plug in labs Ouest team, before appearing on the platform.

Thematics

Current thematics

  • Health and Nutrition
    • Food ingredients
    • New therapeutic approaches
Title

Current content

Laboratory of Cell Physiology
Type

Current content

Research unit
Description

Current content

The scientific project of our laboratory encompasses fundamental aspects of ion channels’ biology with a particular focus on the consequences of their dysregulation during the development of prostate, pancreatic and colon cancers. Our experimental approaches include electrophysiology, imaging, biochemistry, molecular biology and flow cytometry techniques, validated in novel in vivo models. Our understanding of ion channels and some associated inflammasomes in pathological conditions allows us to develop biomarkers and innovative therapeutic approaches for a better management of "oncochannelopathies". Given the molecular complexity, tumor heterogeneity as well as resistance to anti-cancer therapies, we propose a strategy focused on ion channels and associated inflammasomes. This strategy has three components: (1) Identify a molecular "signature" that can be used for the development of a diagnostic/prognostic test for efficacy/toxicity to locoregional therapies in close collaboration with clinicians. (2) Develop the visualization and measurement of dynamic alterations of channels and inflammasomes during carcinogenesis. (3) Improve the current personalized medicine strategy against prostate, pancreatic and colorectal cancers.
Contact informations
Contact

Current content

Contact name Email phone number
DESPEGHEL Sandy sandy.despeghel@inserm.fr (+33) 3 20 33 64 23
PREVARSKAYA Natacha secretariat.u1003@inserm.fr (+33) 3 20 33 60 18
URL

Current content

https://phycell.univ-lille.fr/

Address

Current content

Bâtiment SN3, Avenue Paul Langevin
Campus Cité Scientifique, Université de Lille
59655 VILLENEUVE D’ASCQ
Keywords

Current content

Cancer, Calcium, Ion channels, Prostate, Pancreas, Resistance to chemotherapy, Carcinogenesis, Metastases, Inflammasome
Our search domains
Skills

Current content

• Ion channels • Single channels • Ion channel pharmacology • Intracellular signaling • Calcium signaling • Prostate cancer • Pancreatic cancer • Resistance to chemotherapy
Workforce

Current content

Nombre de chercheurs : 22
Nombre de doctorants :
Nombre de personnel d'appui à la recherche : 8
Effectif total : 47
Example(s) of projects

Current content

• Role of ion channels in tumor initiation and progression. • TRPV6 regulation and targeting in cancer. • Calcium-permeable channels in cancer-related senescence.
Example(s) of publications

Current content

• TRPM8 inhibits endothelial cell migration via a non-channel function by trapping the small GTPase Rap1. Genova T, Grolez GP, Camillo C, Bernardini M, Bokhobza A, Richard E, Scianna M, Lemonnier L, Valdembri D, Munaron L, Philips MR, Mattot V, Serini G, Prevarskaya N, Gkika D, Pla AF. J Cell Biol. 2017 Jul 3;216(7):2107-2130. doi: 10.1083/jcb.201506024. 🡭 • The calcium-cancer signalling nexus. Monteith GR, Prevarskaya N, Roberts-Thomson SJ. Nat Rev Cancer. 2017 Jun;17(6):367-380. doi: 10.1038/nrc.2017.18. 🡭 • Epidermal TRPM8 channel isoform controls the balance between keratinocyte proliferation and differentiation in a cold-dependent manner. Bidaux G, Borowiec AS, Gordienko D, Beck B, Shapovalov GG, Lemonnier L, Flourakis M, Vandenberghe M, Slomianny C, Dewailly E, Delcourt P, Desruelles E, Ritaine A, Polakowska R, Lesage J, Chami M, Skryma R, Prevarskaya N. Proc Natl Acad Sci U S A. 2015 Jun 30;112(26):E3345-54. doi: 10.1073/pnas.1423357112. 🡭 • TRP channel-associated factors are a novel protein family that regulates TRPM8 trafficking and activity. Gkika D, Lemonnier L, Shapovalov G, Gordienko D, Poux C, Bernardini M, Bokhobza A, Bidaux G, Degerny C, Verreman K, Guarmit B, Benahmed M, de Launoit Y, Bindels RJ, Fiorio Pla A, Prevarskaya N. J Cell Biol. 2015 Jan 5;208(1):89-107. doi: 10.1083/jcb.201402076. 🡭 • Remodeling of channel-forming ORAI proteins determines an oncogenic switch in prostate cancer. Dubois C, Vanden Abeele F, Lehen'kyi V, Gkika D, Guarmit B, Lepage G, Slomianny C, Borowiec AS, Bidaux G, Benahmed M, Shuba Y, Prevarskaya N. Cancer Cell. 2014 Jul 14;26(1):19-32. doi: 10.1016/j.ccr.2014.04.025. 🡭
Collaborations/Partners/Scientific clients

Current content

National: University de Valenciennes et du Hainaut-Cambresis, Institut Pasteur Lille, Centrale Lille, INSA Lyon, University Claude Bernard Lyon, CHU Lille, University of Paris, University of Tours, University of Grenoble Alpes, University of Clermont Auvergne International: Institute of Physiology Bogomoletz (Ukraine), University of Turin (Italy), University of Münster (Germany)
Collaborations/Partners/Private Clients

Current content

Services offers
Training offers

Current content

Services offers

Current content

• Electrophysiological study of ion channels (whole cell, single channels...) • Screening of pharmacological molecules and antibodies modulating ion channels • Study of signaling pathways • Calcium imaging
Consulting services

Current content

We provide consulting services related to all our areas of expertise.
Equipments
Equipements spécifiques

Current content

  • Confocal microscope
    LSM 780/ Zeiss
  • Confocal microscope
    LSM 700/ Zeiss
  • Calcium imaging station
    Eclipse Ti + DG4/ Nikon
  • Small animal imaging station
    Bruker
  • Cellular electrophysiology system
    Port-A-Patch/ Nanion
  • Automated device for the preparation of giant unilamellar vesicles (GUV)
    VESICLE PREP PRO/ Nanion
  • Patch-clamp platform
  • ultracentrifuge
    Optima XPN/ Beckman Coulter
Ecosystem
Affiliated institutions / organisations

Current content

  • Inserm
  • University of Lille
Établissement(s) partenaire(s)

Current content

Université catholique de Lille
Unit(s) of attachment

Current content

Groups/Networks/Federations

Current content

GIS ONCOLille ITN pHioniC
Doctoral schools

Current content

Equipex/EquipEx+/ESR

Current content

Competitive cluster

Current content

Regional strategic areas of activity

Current content

  • Health and Nutrition
  • Food ingredients
  • New therapeutic approaches
Personal informations
Back to top