• Health and Nutrition

Translational Research Laboratory for Diabetes (RTD)

Research unit - U 1190

Description

Our laboratory focuses on translational research in diabetes and in particular on human islet transplantation and metabolic surgery. Our research objective is to develop innovative therapies for severe forms of diabetes and their clinical application. Our main research axes are: (i) human islet transplantation for the treatment of type 1 diabetes and (ii) treatment of type 2 diabetes by metabolic surgery.

Contacts

  • Gianni Pasquetti
  • François Pattou
    Director

Informations

1 place de Verdun
Faculté de médecine - Pôle recherche, Campus Santé, Université de Lille
59045 LILLE

https://translational-research-diabetes.com/

Translational Research Laboratory for Diabetes

Effectif

Effectif total : 41

Personnel de recherche : 12

Personnel d'appui à la recherche : 21

Expertises

Skills

• Obesity
• Gastric Bypass
• Glucose transporters
• Induced pluripotent stem cells
• Human embryonic stem cells
• Islet production and transplantation
• Metabolic surgery

Example(s) of projects

• Study of the efficacy of combination therapy (selective SGLT2 inhibitor dapagliflozin + GLP-1 analogue) to improve glycemic control.
• Optimization of pancreatic islet transplantation by ex vivo hypothermic perfusion of human pancreas.
• Involvement of Treg autoimmune reactivation in type 1 diabetes after clinical islet transplantation.
• Regulation of lysosomal function by nutrients in type 2 diabetes.
• Combining low-dose sulfonylureas with the SGLT2 inhibitor dapagliflozin to improve glycemic control.

Example(s) of publications

See the full list of our publications here.

Collaborations/Partners/Scientific clients

National:
Heart and Arteries Foundation, Francophone Diabetes Research Foundation, Francophone Diabetes Society, Association for Diabetes Research

International:
European Foundation for the Study of Diabetes, Catholic University of Louvain (Belgium), Columbia University (United States)

Collaborations/Partners/Private Clients

Cousin Biotech

Services offers

Applications sectors

  • Biotechnology
  • Health / wellness
  • Science / Research

Services provided

• Preparation of human islets of Langerhans
• Production of beta-pancreatic cells from induced pluripotent stem cells (iPS) and human embryonic stem cells
• Assistance in protocol development
• Development of cell therapies

Consulting services

We provide consulting services related to all our areas of expertise.

Equipments

We possess a wide range of equipment regrouped within the internal and external technological platforms:
- Biotherapy platform of 250 m² for the preparation of cell therapies.
- Biochemistry platform
- Histology platform
- Imaging platform
- Epigenetics platform
- Immunophenotyping platform

Our laboratory is fully equipped for project development. Our facilities include:
- Controlled Atmosphere Zone (CAZ)
- Cell culture machines
- Perfusion and perifusion machines
- Microscopes (confocal, inverse, fluorescent, motorized stage)
- Analyzers (CO2, O2, proteins, RNA)
- Incubators, circulating coolers, cryopreservers
- Centrifuges
- Plate readers, assays
- Rodent anesthesia station...

Biological materials

• Human pancreatic islets, cell lines (INS1E, MIN6, EndoBH5...)
• Plasmids, siRNA
• Antibodies
• Murine models (knock-out, QIVIPA, high fat diet, rats with gastric bypass), pigs
• Biological collections (frozen human islet pellets, human islet RNA, murine and pig tissues (liver, pancreas, intestine, kidney, muscle, fat), human tissues (liver, pancreas, intestine, fat)

Ecosystem

Affiliated institutions / organisations

CHU de Lille
Inserm
University of Lille
Institut Pasteur de Lille

Doctoral schools

Regional strategic areas of activity

  • Health and Nutrition
    • New therapeutic approaches

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Thematics

Current thematics

  • Health and Nutrition
    • New therapeutic approaches
Title

Current content

Translational Research Laboratory for Diabetes
Type

Current content

Research unit
Description

Current content

Our laboratory focuses on translational research in diabetes and in particular on human islet transplantation and metabolic surgery. Our research objective is to develop innovative therapies for severe forms of diabetes and their clinical application. Our main research axes are: (i) human islet transplantation for the treatment of type 1 diabetes and (ii) treatment of type 2 diabetes by metabolic surgery.
Contact informations
Contact

Current content

Contact name Email phone number
PASQUETTI Gianni gianni.pasquetti@univ-lille.fr (+33) 3 20 62 69 63
PATTOU François contact@translational-research-diabetes.com (+33) 3 20 44 44 11
URL

Current content

https://translational-research-diabetes.com/

Address

Current content

1 place de Verdun
Faculté de médecine - Pôle recherche, Campus Santé, Université de Lille
59045 LILLE
Keywords

Current content

Diabetes, metabolic diseases, cardiovascular diseases, diabetes biotherapy, obesity, transplantation, metabolism, blood sugar regulation
Our search domains
Skills

Current content

• Obesity • Gastric Bypass • Glucose transporters • Induced pluripotent stem cells • Human embryonic stem cells • Islet production and transplantation • Metabolic surgery
Workforce

Current content

Nombre de chercheurs : 12
Nombre de doctorants :
Nombre de personnel d'appui à la recherche : 21
Effectif total : 41
Example(s) of projects

Current content

• Study of the efficacy of combination therapy (selective SGLT2 inhibitor dapagliflozin + GLP-1 analogue) to improve glycemic control. • Optimization of pancreatic islet transplantation by ex vivo hypothermic perfusion of human pancreas. • Involvement of Treg autoimmune reactivation in type 1 diabetes after clinical islet transplantation. • Regulation of lysosomal function by nutrients in type 2 diabetes. • Combining low-dose sulfonylureas with the SGLT2 inhibitor dapagliflozin to improve glycemic control.
Example(s) of publications

Current content

See the full list of our publications here.
Collaborations/Partners/Scientific clients

Current content

National: Heart and Arteries Foundation, Francophone Diabetes Research Foundation, Francophone Diabetes Society, Association for Diabetes Research International: European Foundation for the Study of Diabetes, Catholic University of Louvain (Belgium), Columbia University (United States)
Collaborations/Partners/Private Clients

Current content

Cousin Biotech
Services offers
Training offers

Current content

Services offers

Current content

• Preparation of human islets of Langerhans • Production of beta-pancreatic cells from induced pluripotent stem cells (iPS) and human embryonic stem cells • Assistance in protocol development • Development of cell therapies
Consulting services

Current content

We provide consulting services related to all our areas of expertise.
Equipments
Equipements spécifiques

Current content

  • Cell processing workstation
    LEAP/ Cyntellect
  • Immunohistochemistry automaton
    Autostainer Plus/ Dako Cytomation
  • Automated microbiological monitoring system
    Bactalert/ Biomerieux
  • Cell culture machine
    Prisma/ Macopharma
  • Perfusion machine
    Suprafusion 2500/ Brandel
  • Thermocycler
    Verity/ Applied Biosystems
  • Optical particle counter
    Aerotrack/ TSI
  • Cryoconserver
    GT70/ Air Liquide
  • RNA Bioanalyzer
    Experion/ Biorad
  • qPCR thermocycler
    CFX96/ Biorad
Ecosystem
Affiliated institutions / organisations

Current content

  • CHU de Lille
  • Inserm
  • University of Lille
  • Institut Pasteur de Lille
Établissement(s) partenaire(s)

Current content

Unit(s) of attachment

Current content

Groups/Networks/Federations

Current content

Doctoral schools

Current content

Equipex/EquipEx+/ESR

Current content

Competitive cluster

Current content

Regional strategic areas of activity

Current content

  • Health and Nutrition
  • New therapeutic approaches
Personal informations
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