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Health and Nutrition
Mass spectrometry for Omics
Platform
Our platform is specialized in proteomics and mass spectrometry for biological and clinical research applications. In particular, our platform offers a wide range of analyses and a high level of expertise in the fields of microproteomics, molecular imaging by mass spectrometry, the study of protein-protein interactions (PPI) and in vivo mass spectrometry. We have extensive experience and state-of-the-art instrumentation for sample preparation, imaging analysis by mass spectrometry or spatial proteomics and data analysis. We are open to academic, clinical and industrial collaborations, and propose both methodological / technological development and services.
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Michel Salzet
Scientific manager -
Fabrice Bray
Contact -
Isabelle Fournier
Scientific manager
Bât SN3 et Bât C4
Campus Cité Scientifique, Université de Lille
59655 VILLENEUVE D'ASCQ
https://laboratoire-prism.fr/index.php/platforms/clic-imaging
Chiffres clés
Number of regional projects per year = 19Number of national projects per year = 8
Number of international projects per year = 8
Number of industrial projects = 11
Participation in European projects = 3
Number of analyses per year : 6000
Stored data = 200 TB
Effectif
Effectif total : 10
Skills
• Spatial Proteomics
• Microproteomics
• Omics
• Mass Spectrometry
• Molecular Imaging by Mass Spectrometry
• In vivo mass spectrometry
• Protein analysis
• Protein-protein interactions
Example(s) of projects
• Search for diagnostic and prognostic markers of pancreatic cancer in mouse models - mass spectrometry mapping of neoplastic lesions (MALDI-MSI) combined with microproteomics to identify new progression markers.
• Comparative proteomic and transcriptomic analysis of follistatin-induced skeletal muscle hypertrophy
• Analysis of the local microbiota in breast cancer - localized microproteomic analysis of 12 patients
• Proteomic study of the effect of cell passage number and stimulation conditions in primary human fibroblasts
Collaborations/Partners/Scientific clients
University of Lyon, Univ. of Nice, Institute of Regenerative Medicine and Biotherapies, Montpellier, University of Rouen, University of the Littoral, University of Lorraine, University of Southern Brittany, Centre for Research and Restoration of the Museums of France.
International:
University of Montreal, Canada; Academy of Sciences Bratislava, Slovakia; Università del Salento, Lecce, Italy; Univ. Mons, Belgium;
University of Florida, USA; University of Ioannina, Greece; Luxembourg Institute of Health, Luxembourg; University of Twente, Netherlands; Gent University, Belgium
Collaborations/Partners/Private Clients
Applications sectors
- Health / wellness
- Agri-food industry
- Art / Culture
- Cosmetics
- Science / Research
Services provided
• Separation (1D, 2D, Gel Free, 2D-DIGE, various chromatographies)
• Identification and relative quantification of proteins (exact mass, HR-MS coupling) by different non-targeted approaches (DIA, DDA) with (iTRAQ, SILAC, AQUA) or without labeling by Bottom-Up, Middle-Up/Middle-Down and Top-Down strategies
• Absolute protein quantification by targeted approaches (SRM, MRM, PRM)
• Analysis of intact proteins
• De novo protein sequencing by deploying different ion activation methods (ETC, ECD, IRMPD)
• Analysis of post-translational modifications (phosphorylation, glycosylation...) and access to ion mobility
• Enrichment in phospho or glycopeptides / proteins
• Identification of proteins by enzymatic digestion from various samples (powder, liquid, bone, tissue, plasma, gel...)
• Analysis of samples from cultural heritage, archaeology or paleontology
• Analysis of lipids such as triacylglycerols or metabolomics
• Mass spectrometry imaging (metabolites, lipids, peptides, proteins) from frozen or FFPE tissues
• Spatial proteomics (SpatiaXomics) by microextraction (LMJ LESA technology)
• Data processing and interpretation (protein arrays, GO terms)
Consulting services
- for sample preparation (microspray system, piezoelectric microdeposition system, microextraction system, manual sublimation system, antigen unmasking system...),
- for proteomic and mass spectrometry analysis (high throughput MALDI-TOF imaging system, high spectral resolution MALDI system, ESI Q-Orbitrap Q-Exactive system...)
- for data analysis (Data server, Proteome Destroyer, computing station with Nvidia Tesla K20c GPU, FlexImaging 4, SCiLS Lab, data processing software...).
Equipments
| Nom | Modèle | Marque |
|---|---|---|
| MALDI-TOF | RapiFlex/ | Bruker |
| MALDI/ESI LTQ | Orbitrap XL / | Thermo Scientific |
| MS4Omics - MALDI-TOF/TOF | Ultraflex II / 4800/ | Bruler Daltonics / Absciex |
| ESI Q-Orbitrap | Q-Exactive / Q-Exactive plus/ | Thermo Scientific |
| Nano-UHPLC | EASY-nLC 1000 / U3000 RSLC/ | Thermo Scientific |
| FT-ICR | SolariX XR 9.4 Tesla/ | Bruker |
| MS4Omics - CZE ProteoLab | PA 800/ | Beckmann Coulter |
| Microspray system | / | |
| Piezoelectric microdeposit system | CHIP1000 / | Shimadzu |
| Microextraction system | Triversa Nanomate LESA plus/ | Advion |
Affiliated institutions / organisations
Unit(s) of attachment
Labellisations
Regional strategic areas of activity
- Health and Nutrition