• Health and Nutrition

High throughput and high content screening platform (ARIADNE CRIBLAGE)

Platform

Description

Our platform combines the know-how and equipment dedicated to small molecule and siRNA screening. Our equipment allows the complete or partial automation of the screening steps, from the plating of molecules from chemical or siRNA libraries, to the reading of results. We have access to chemical libraries and siRNA/miRNA libraries, and develop miniaturized (96, 384 well plates) and automated high throughput assays for in vitro, in bacterio, or in cellulo, target or phenotypic screening. The proposed tests include enzymatic assays, protein-protein interactions, cell-based assays, TSA, target engagement monitoring by CETSA and many others. Within the framework of a partnership agreement, projects can be pursued with Ariadne-Criblage experts towards target characterization and modulator optimization: target identification and engagement, medicinal chemistry, physicochemical properties measurement, ADME and pharmacokinetic studies in rodents. We are open to all academic and industrial collaborations and propose training and services in line with our expertise.

Contacts

  • Florence Leroux
    Scientific manager
  • Alexandre Vandeputte
    Technical manager

Informations

Bâtiment Institut de Biologie de Lille et Bâtiment Debeyre, 1 Rue du Professeur Calmette
59000 LILLE

https://ums-plbs.univ-lille.fr/les-plateformes-constitutives/ariadne-criblage

Chiffres clés

• 60% of time dedicated to innovation
• 3000 hours / year of use of the cell screening platform
• 10 new users / year
• 40 publications over the last five years
• 43 new study models over five years

Effectif

Effectif total : 2

Expertises

Skills

• High throughput testing
• Automated testing
• High content and high throughput screening
• Screening automation
• Automated confocal microscopy
• In vitro, in bacterio, or in cellulo screening, on targets or phenotypic
• Target characterization and modulator optimization

Collaborations/Partners/Scientific clients

National:
Institut Pasteur Paris; Institut Necker Paris; Paris Orsay; Université Paris Sud/ Institut Galien Institut Polytechnique de Paris Université Angers

International:
Sciensano (Belgium), Paul Langerhans Institute Dresden (Germany)

Collaborations/Partners/Private Clients

Bioversys, Apteeus, GSK

Services offers

Applications sectors

  • Biotechnology
  • Health / wellness
  • Science / Research

Services provided

• Development of miniaturized (96, 384 well plates) and automated high throughput tests for in vitro, in bacterio, or in cellulo, target or phenotypic screening
• High throughput and high content screening (HTS, HCS) in different containment environments (biosafety level 1 to 3 laboratories)
• Enzymatic assays, phenotypic or target cell assays, protein-protein interactions in vitro or in cellulo, Thermal Shift Assay ...
• Multimode reading (Fluorescence, fluorescence polarization, TR-FRET, BRET, Alpha ...)
• Confocal microscopy and quantitative and automated image analysis

Training offers

HCS BSL2 and BSL3 training

Consulting services

We provide consulting services related to all our areas of expertise.

Equipments

Our platform hosts various biology programs (microbiology, toxicology, oncology, neurology, etc.) and includes a wide range of equipment:
• robotic assemblies for screening (BSL 2 and 3)
• high throughput confocal microscopy
• acoustic dispenser
• liquid dispensers
• robotic arms
• fluorescence readers
• automated incubators
• PCR systems

Equipments

Nom Modèle Marque
Automated microscope InCell Analyzer 6000/ GE Healthcare
High content screening system Opera/ PerkinElemer
Liquid handler Echo 550/ Labcyte

Ecosystem

Affiliated institutions / organisations

CHU de Lille
Inserm
CNRS

Unit(s) of attachment

Groups/Networks/Federations

GIS IBiSA
IR ChemBioFrance
GDR ChemBioScreen
GDR ChémoBiologie

Labellisations

Regional strategic areas of activity

  • Health and Nutrition
    • Food ingredients
    • New therapeutic approaches

Request for modification

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Thematics

Current thematics

  • Health and Nutrition
    • Food ingredients
    • New therapeutic approaches
Title

Current content

High throughput and high content screening platform
Type

Current content

Plateforme de services
Description

Current content

Our platform combines the know-how and equipment dedicated to small molecule and siRNA screening. Our equipment allows the complete or partial automation of the screening steps, from the plating of molecules from chemical or siRNA libraries, to the reading of results. We have access to chemical libraries and siRNA/miRNA libraries, and develop miniaturized (96, 384 well plates) and automated high throughput assays for in vitro, in bacterio, or in cellulo, target or phenotypic screening. The proposed tests include enzymatic assays, protein-protein interactions, cell-based assays, TSA, target engagement monitoring by CETSA and many others. Within the framework of a partnership agreement, projects can be pursued with Ariadne-Criblage experts towards target characterization and modulator optimization: target identification and engagement, medicinal chemistry, physicochemical properties measurement, ADME and pharmacokinetic studies in rodents. We are open to all academic and industrial collaborations and propose training and services in line with our expertise.
Contact informations
Contact

Current content

Contact name Email phone number
LEROUX Florence florence.leroux@pasteur-lille.fr
VANDEPUTTE Alexandre alexandre.vandeputte@univ-lille.fr
URL

Current content

https://ums-plbs.univ-lille.fr/les-plateformes-constitutives/ariadne-criblage

Address

Current content

Bâtiment Institut de Biologie de Lille et Bâtiment Debeyre, 1 Rue du Professeur Calmette

59000 LILLE
Keywords

Current content

High content and high throughput screening, screening automation, chemical library, siRNA / miRNA, cell assays, enzymatic assays, ADME, pharmacokinetics, IBiSA
Our search domains
Skills

Current content

• High throughput testing • Automated testing • High content and high throughput screening • Screening automation • Automated confocal microscopy • In vitro, in bacterio, or in cellulo screening, on targets or phenotypic • Target characterization and modulator optimization
Key data

Current content

• 60% of time dedicated to innovation • 3000 hours / year of use of the cell screening platform • 10 new users / year • 40 publications over the last five years • 43 new study models over five years
Workforce

Current content

Effectif total : 2
Example(s) of projects

Current content

Example(s) of publications

Current content

Collaborations/Partners/Scientific clients

Current content

National: Institut Pasteur Paris; Institut Necker Paris; Paris Orsay; Université Paris Sud/ Institut Galien Institut Polytechnique de Paris Université Angers International: Sciensano (Belgium), Paul Langerhans Institute Dresden (Germany)
Collaborations/Partners/Private Clients

Current content

Bioversys, Apteeus, GSK
Services offers
Training offers

Current content

HCS BSL2 and BSL3 training
Services offers

Current content

• Development of miniaturized (96, 384 well plates) and automated high throughput tests for in vitro, in bacterio, or in cellulo, target or phenotypic screening • High throughput and high content screening (HTS, HCS) in different containment environments (biosafety level 1 to 3 laboratories) • Enzymatic assays, phenotypic or target cell assays, protein-protein interactions in vitro or in cellulo, Thermal Shift Assay ... • Multimode reading (Fluorescence, fluorescence polarization, TR-FRET, BRET, Alpha ...) • Confocal microscopy and quantitative and automated image analysis
Consulting services

Current content

We provide consulting services related to all our areas of expertise.
Equipments
Equipements spécifiques

Current content

  • Automated microscope
    InCell Analyzer 6000/ GE Healthcare
  • High content screening system
    Opera/ PerkinElemer
  • Liquid handler
    Echo 550/ Labcyte
Ecosystem
Affiliated institutions / organisations

Current content

  • University of Lille
  • CHU de Lille
  • Inserm
  • CNRS
Établissement(s) partenaire(s)

Current content

Unit(s) of attachment

Current content

  • Center for Infection & Immunity of Lille
Groups/Networks/Federations

Current content

GIS IBiSA IR ChemBioFrance GDR ChemBioScreen GDR ChémoBiologie
Competitive cluster

Current content

Regional strategic areas of activity

Current content

  • Health and Nutrition
  • Food ingredients
  • New therapeutic approaches
Labelling

Current content

Personal informations
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